Seed-X increases probability of success in breeding superior quality hybrid tomatoes

Tomatech, a dynamic and innovative tomato seed breeding company, and Seed-X, are collaborating to improve and accelerate the breeding of superior quality hybrid tomatoes.

Pairing Seed-X’s advanced computer vision and AI-powered Genetic Traits Detection technology with Tomatech’s innovative, superior quality, hybrid tomato program, we have successfully demonstrated a significant increase in the probability of achieving breeding targets and reducing time to market.

During the collaboration, breeding populations of two parental lines, P1, P2, and their F1, and F2, were grown together in the same greenhouse. The parental lines were selected to represent a wide variation of biotic traits, and fruit quality traits such as fruit color, size and taste - controlled by single or multi-genes.

Around 400 F2 plants were characterized for the different traits, using PCR markers, and characterized by Tomatech professional breeders according to standard practice. Individual plants from both sides of the curve (tails), for several different traits were selected. Next, hundreds of seeds from each of the selected plants were produced. All seeds were processed according to standard practice.

Using Seed-X’s AI-powered GeNee™, the seeds images were ‘captured’ and used to train the system and develop a unique fingerprint for each phenotype. These fingerprints were used to screen thousands of F2 seeds, and to select for the phenotype of interest. The selected seeds were planted in the field, and their phenotypic results were compared to a random F2 population.

The selected seeds were significantly enriched for the selected traits, compared to the random population. For example, 17% of the selected seeds had superior taste compared to around 7% in the random population.

When looking at fruit color – the percentage of purple color traits in the selected seeds was 28% compared to 7% in the random population, and the trait of fruit size in the selected seeds were 41% large-sized fruit compared to 25% in the random population.

We successfully demonstrated the enrichment of populations with complicated traits.